Vol. 7, Issue 8, Part B (2025)

The Y-chromosome microsatellite DNA marker is used to understand the polymorphism of this marker in native cattle from Tamil Nadu, as the Y-chromosome marker serves as an analog line for males, while the mitochondrial DNA (mtDNA) line is relevant for females. The study involved 25 unrelated male animals from each breed, resulting in a total of 100 samples across the four breeds. The maximum number of alleles was two alleles identified at each Y-microsatellite locus, while the average allele count across populations was 1.1, and the total number of alleles was relatively low (ranging from 1 to 2 alleles). The heterozygosity values for seven breeds ranged from 0 to 53%. The INRA 062 locus had the highest heterozygosity value (53%) among the microsatellite loci in the Pullikulam cattle population, which are local cattle from southern part of Tamil Nadu. The PIC values for all microsatellites ranged from 0.10 to 0.29, with the highest value recorded in Umblacherry cattle.