Vol. 7, Issue 8, Part B (2025)

Endophytic microorganisms residing within plant tissues play a crucial role in enhancing plant growth and resilience against environmental stresses. This study aimed to isolate endophytic microorganisms from Parthenium heterophorias L. and Portlike oleracea L. to evaluate their potential for promoting plant growth. Healthy plant samples were collected and surface sterilization techniques were employed to isolate true endophytes. A diverse range of bacterial endophytes were isolated, with several demonstrating the production of plant growth-promoting substances such as indole-3-acetic acid (IAA), siderophores, and phosphate solubilization. Additionally, some isolates exhibited antagonistic activity against common plant pathogens. Key isolates, particularly Pseudomonas aeruginosa strains PoR4(a), PoR5 and PoR5(a), exhibited superior plant growth-promoting activities and disease resistance. These findings underscore the potential of these endophytic microorganisms as sustainable bio-inoculants in agriculture, warranting further research into their broader applications and secondary metabolite production. The study highlights the potential of endophytic microorganisms from invasive plants as bio- inoculants to enhance agricultural productivity and sustainability. Further research is recommended to explore their application in various crops and environmental conditions.