Vol. 7, Issue 6, Part E (2025)

Stress is an unavoidable aspect of life, and the impacts on biological systems are of great scientific interest. Oxidative stress, defined as an imbalance between reactive oxygen species (ROS) generation and antioxidant defenses, has been related to a variety of illnesses, including neurological disorders, cardiovascular disease, and cancer. Antioxidants serve an important function in reducing oxidative stress by neutralizing ROS and maintaining cellular homeostasis. Because of its short life cycle, genetic conservation with humans, and sophisticated genetic manipulation methods, Drosophila melanogaster has become a potent model organism for researching oxidative stress and assessing the effectiveness of antioxidants. This review discusses the mechanics of oxidative stress, the function of antioxidants in cellular defense, and the advantages of using Drosophila in oxidative stress studies. Antioxidant activity in Drosophila is assessed using a variety of procedures, including survival experiments, behavioral analysis, and genetic techniques. The findings highlight the effectiveness of plant-derived antioxidants in reducing oxidative damage, as well as the consequences for human health. Drosophila is an effective and ethical model for early drug screening, which aids in the discovery of new therapeutic compounds for oxidative stress-related disorders.