Vol. 7, Issue 3, Part A (2025)

Proteins make up more than half the dry weight of the cells. They contribute tothe structure of a cell and are obliged for cellular functions analogous as catalysisand molecular recognition. Also, the phenotypes are linked by the proteinsnot by the genes. The disquisition now a day is going in a direction towards thecharacterization of the proteome, or the whole set of the proteins set up in thecell, kerchief or organism. To understand how cell works, there is a demand ofidentification and characterization of the proteins. In short accurate protein quantitation is essential to protein studies in a multitude of disquisition motifs. Sothat a wide array of different protein assays has been developed to quantify andidentify both complex mixtures of proteins as well as a single type of proteins. Total protein quantitation styles include Bradford assay, BCA assay and Absorbance at 280m. Kjeldahl system is used to know the chance of proteinpresent in the sample. There are multitudinous ways for the identification ofproteins, mainly there are two ways performed during design work thatare SDS - runner and Immunoblotting. Estimation of the total proteinattention in bio Medicinals or remedial drugs is truly important. Itgives the information about the stability of the drugs. Stability testing is one ofthe essential ways on the path of drug development. The process determineswhether any changes like physical, chemical or microbiological affect the effectivenessand integrity of the final product. Particular fashion is been distributed todifferent samples depending on the structure of proteins, vacuity ofmorals and the perceptivity of the fashion. The acceptance criteria for all the mentioned ways were achieved.