Vol. 7, Issue 12, Part A (2025)

Tilapia lake virus (TiLV) is a novel virus that causes large scale mortalities in Tilapia farming and is considered as a threat to the global tilapia industry. In the present study, the cell toxicity and antiviral activity of homeopathy medicines against TiLV using SSN1 cell line was investigated. A total of 10 homeopathy medicines including Belladonna 200C, Pyrogenium 200C, Influenzinum 200C, Crotalus horridus 200C, Arsenicum album 200C, Hepar sulphur 200C, Aconitum napellus 200C, Tuberculinum 200C, Bothrops lanceolatus 200C and Nux vomica 200C were used at different concentrations viz, 100 μl /ml, 250 μl /ml, 500μl /ml and 750μl /ml of medium for screening the antiviral activities against TiLV. Among the 10 medicines, 3 medicines Crotalus horridus, Influenzinum and Pyrogenium inhibited the cytopathic effect caused by TiLV in SSN1 cell line and cell toxicity was occurred at 750μl /ml. Further, these three medicines at a concentration of 100 μl/ml of medium were used for virus neutralization study which showed antiviral activity against two different concentrations of virus (10^1.0TCID₅₀ ml^-1 and 10^6.75TCID₅₀ ml^-1). In this study, the concentration of homeopathy medicine at 500μl /ml of the medium showed better effect against TiLV in both virus concentrations of 10^1.0TCID₅₀ ml^-1 and 10^6.75TCID₅₀ ml^-1 when compared to the control group. Our study has revealed that homeopathy medicines have potent antiviral effects in fish virus in cell culture. The finding suggests that homeopathy medicines have the potential as a therapeutic agent in the treatment of TiLV infection.