Vol. 7, Issue 11, Part A (2025)

Micropropagation gives a rapid and reliable approach for large scale production of disease-free plants overcoming the limitations of conventional farming. Sustainable agriculture demands new innovative approaches to increase food productivity while protecting the environment for future generations. The study focuses on two nutraceutically and economically important Brassicaceae crops: Brussels sprouts (Brassica oleracea var. gemmifera) and Chinese cabbage (Brassica rapa subsp. pekinensis), evaluated for in vitro growth in different media (Morishige and Skoog-MS, B5, and White medium) along with the effects of plant growth regulators.
Uniform growth of plantlets was observed throughout the study period. Growth parameters, including shoot length, number of nodes and callus formation, were recorded over 60 days. Results showed that plantlet growth was enhanced with PGRs, and callus formation was observed in medium containing 2,4-D. The synergistic combination of 2 ppm BAP and 1 ppm NAA in MS medium led to the most significant shoot and node proliferation, producing an average of 6 shoots in Brussels sprouts and 5 nodes in Chinese cabbage by Day 60.
Commercial micropropagation of these crops makes large scale cultivation of crops a possibility, which promotes sustainability. agriculture and the accessibility to nutraceutical-rich vegetables that have been shown to have anticancer and immunomodulatory properties.