Vol. 7, Issue 10, Part B (2025)

It is becoming more widely acknowledged that leptospirosis, a zoonosis of global importance, is a major but underdiagnosed cause of bovine reproductive failure. The purpose of this study was to look into the role of Leptospira in causing abortions, stillbirths and recurring breeding problems in cattle in Kerala. A combination of serological, molecular and cultural techniques were used to sample and assess a total of 20 animals. Five animals tested positive for Leptospira DNA by polymerase chain reaction (PCR) targeting the LipL32 gene, while two animals tested seropositive in microscopic agglutination test (MAT). Leptospira were successfully isolated from two samples (liver and blood). The results emphasise how crucial it is to use a variety of diagnostic techniques in order to accurately identify both active infections and carrier states.