Vol. 7, Issue 1, Part A (2025)

Toxicological analysis is critical for diagnosing poisoning, assessing food and feed safety, and monitoring environmental contamination. This discussion highlights the importance of a multidisciplinary approach in toxicological investigations, emphasizing advancements in analytical and diagnostic techniques. Key aspects include proper sample collection, handling, and storage to ensure the reliability of results. Microscopic methods, integrated with chemical analysis, and innovative imaging techniques such as holographic imaging, enhance the detection of micro- and nanoparticles. Chromatographic techniques like thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are widely employed for qualitative and quantitative analyses of toxic substances. Additionally, proteomic approaches, particularly toxicoproteomics, provide valuable insights into cellular responses to toxic agents and help identify biomarkers of toxicity. Mycotoxins, pesticide residues, pharmaceutical contaminants, and foodborne pathogens are identified as significant contributors to toxicity in humans and animals. Persistent pesticides, such as organochlorines, pose long-term risks due to their slow degradation. The integration of proteomics, chromatography, and microscopy enables comprehensive toxicological assessments, paving the way for better diagnostic, therapeutic, and preventive measures. This multidisciplinary framework ensures the effective identification and management of toxicants, contributing to improved health and safety standards.