Dr. Tapash Kumar Bhowmik, Md. Mahbubur Rahman, Anamika Paul and Tripa Paul
Micropropagation of Dendrobium crepidatum an epiphytic medicinal orchid, can be employed as ex situ conservation. Different concentrations and combinations of PGRs, auxins (IAA, IBA, NAA, Picloram) and cytokinins (BAP, Kinetin) supplemented MS media were used for MSBs or PLBs development from in vitro raised nodal and leaf segments of germinated plantlets. In case of nodal segments, the highest percentage of MSBs per segment was induced through organogenesis on MS medium supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA and the rate was 77.33±1.54 percent within the minimum required time 4.74±0.17 weeks and the average number of induced MSBs was 6.8±0.37. On the other hand, embryogenesis occurred in leaf segment and the highest percentage of greenish PLBs was induced on MS medium fortified with 3.0 mg/l BAP and 1.5 mg/l IAA and the maximum rate was 65.33±1.44 percent within the lowest requisite period 4.74±0.17 weeks. After 30 days of culture, the highest rate was 3.66±0.04 cm on liquid condition; whereas on solid media the utmost rate was 3.57±0.04 cm. It was revealed that MS medium fortified with 2.0 mg/l Kn and 1.2 mg/l NAA was the most effective for the elongation of both solid and liquid culture for development of MSBs from nodal segments. MS medium fortified with 1.0 mg/l IAA and 1.0 mg/l IBA was found best for effective induction and growth of roots on elongated shoot buds culture where the increased number of roots per seedling was 5.33±0.33 with the increased length of 3.39±0.05 cm.
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