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International Journal of Biology Sciences

Vol. 5, Issue 1, Part B (2023)

Isolation and antimicrobial sensitivity test of Salmonella isolates from selected intensive dairy farms in Bishoftu town, Central Ethiopia

Author(s):

Ephrem Shimelis

Abstract:

Salmonella is one of persisting foodborne pathogenic infection particularly food of animal origin including dairy cattle products. Accoringly a cross-sectional study was conducted from July to September 2021 to determine the prevalence and antibiotics resistant of Salmonella in Bishoftu town. To conduct the study 61 samples were collected randomly from the individual lactating cows. The Salmonella was recovered from the samples; conventional culture characterization of Salmonella was conducted by pre-enrichment on the buffered peptone water broth and enrichment in Rappaport- Vassiliadis (RV) broth, subsequently isolation was done on selective XLD and biochemical confirmation of Salmonella isolates. Finally, antibiotic sensitivity tests against the identified isolate were done by using eight drugs. Salmonella was isolated from 13/61 (21.3%, 95% CI: 11.3-31.3) of the total samples. Out of the 13 Salmonella isolates, 11/49 (22.4% 95% CI: 11.4-33.4) and 2/121 (16.7% 95% CI: 4.77-38.17) were isolated from milk cows and milk container respectively with no significance (P-value = 0.362). The isolates were susceptible to Ciprofloxacin, Penicillin and Vancomycin) were susceptible to the isolates, while, Streptomycin, Ampicillin, Tetracycline Rifampicillin and Cloxacillin) were resistant to the isolates. The effectiveness of such drugs like ciprofloxacin may be because they are not widely used in countries like Ethiopia and other African countries for animal’s treatment. 100% rate of resistant was recorded to Ampicillin, Chloramphenicol, Cloxacillin, Streptomycin and Amoxicillin. The study indicated the prevalence of Salmonella with high proportion of multiple antimicrobial resistance to the commercial used in the study area. Therefore, the government, farmers and other stakeholders should give concern for dairy farm hygiene and antibiotics uses in dairy farms.

Pages: 109-113  |  443 Views  166 Downloads


International Journal of Biology Sciences
How to cite this article:
Ephrem Shimelis. Isolation and antimicrobial sensitivity test of Salmonella isolates from selected intensive dairy farms in Bishoftu town, Central Ethiopia. Int. J. Biol. Sci. 2023;5(1):109-113. DOI: 10.33545/26649926.2023.v5.i1b.155