Alifa Amalia Izzati, Berlian Putri Ambarningrum, Gunanti Mahasri and Yudi Cahyoko
The main problem that often occurs in grouper cultivation is the appearance of disease attacks caused by pathogens due to water quality that has not been optimal during maintenance. There are common problems in grouper cultivation, that is unstable environmental conditions that cause accumulation of organic matter which can cause fish to be stress, which is characterized by increased blood glucose levels and susceptible to disease. The types of ectoparasites that commonly attack groupers are Trichodina, Cryptocaryon, Zeylanicobdella and Neobenedenia. An alternative to control pathogen attacks on fish is by increasing the body's defense cells of fish using immunostimulants. Zeylanicobdella known to have molecular weight of proteins that are immunogenic, and is thought to increase the activity of cells defense of the fish body as an immunostimulant. This study aims to find out the blood glucose level, blood profile and ectoparasite infestation of cantang grouper fish given a marine leeches Zeylanicobdella on feed. The method in this research used an experimental method with a factorial pattern consisting of two factors. Factor P is the dose given and factor Q is the maintenance time. Statistical test results showed there was an influence (p < 0.01) between the doses of marine leeches Zeylanicobdella on the number of erythrocytes and leukocytes, there was also interaction (p > 0.01) between the doses of marine leeches Zeylanicobdella with different maintenance times to the number of erythrocytes, leukocytes, differential leukocytes including lymparhocytes, neutrophils and monocytes. The results showed that there was a very significant difference between blood glucose levels and ectoparasite infestation of cantang grouper maintenance at different doses and times and there was an interaction effect (p < 0.01). The highest blood glucose level of 67.00 ± 5.148 mg/dL occurred in cantang grouper with a moderate degree of ectoparasite infestation of 36.20 ± 2.387 parasites on day 0 of maintenance with a dose of 0 ppm. The lowest blood glucose level of 28.80 ± 0.837 mg/dL occurred in cantang grouper with a mild degree of ectoparasite infestation, that is 12.60 ± 1.517 parasites on the 28th day of rearing with a dose of 9 ppm. The results showed that the highest of total erythrocytes was at a dose of 7 ppm with a maintenance time of the 21th day are 2,534 × 106 cell/ml. Total Leukocyte was at a dose of 9 ppm with a maintenance time of the 28th day are 1,4902 × 106 cell/ml. The percentage of lymphocytes is the highest with a range of 58-93%, neutrophils range from 5-38% and monocytes range from 1-9%.
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