Muhammad Yaseen, Nazia Ehsan, Syed Makhdoom Hussain and Saima Yousaf
Earthworms are the important natural invertebrate of agro-ecosystem belonged to phylum Annelida and class Oligochaeta and are called as ‘biological engineers. They improve Physical, Chemical and Biological assets of soil. Earthworms provide rich source of protein about 70% and used as medicines for the treatments of numerous diseases. The current study was to examine the special effects/of pesticide deltamethrin/and weedicide glyphosate on DNA damage of earthworm’s specie Lumbricus terrestris by using comet assay in order to calculate deoxyribonucleic acid/DNA damage in the nuclei of cells. Samples of study specie were collected from the soil near to Fisheries ponds of Department of Zoology Wildlife and Fisheries of University of Agriculture Faisalabad and brought to the Biomonitoring Laboratory. A total of seven Experiments including control group were performed in Biomonitoring Lab for analysis of DNA damage. Samples were exposed to soil that was contaminated with 0.000135ml and 0.0029ml concentrations of deltamethrin and 1ml and 3.5ml concentrations of glyphosate also two combined concentrations like 0.0007ml deltamethrin and 1.75ml glyphosate like wise 0.000145ml deltamethrin and 0.5ml glyphosate. Experiment was performed for 90 days and then taken out from experimental containers and preserved in preserving solution. ANOVA as statistical tool give the significant damage on DNA of samples. Similarly, results of comet assay show significant damage in DNA as compare to control group. Least scattered type of comets was formed in most effective treatments and scattered type of comets in control as well as less effective treatments.
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