Vol. 4, Issue 1, Part A (2022)

In the agricultural field, many pesticides are used to control various pests in crops. Pesticides consist of many chemicals which are also harmful to human health and caused environmental pollution. Organophosphates are the most widely used group of pesticides. Profenofos is one of the most largely used organophosphate insecticides on a variety of crops including cotton and vegetables. The World Health Organization classified this compound as moderately hazardous (Toxicity class II). Many microbes are capable of the biodegradation of profenofos from pesticides contaminated soil. In context to the same, in the present study, we used two bacterial strains which are isolated from the pesticides contaminated soil by enrichment culture technique and identified by morphological characteristic and biochemical reactions were found to be Acinetobacter ursingiiDN-4 and SphingomonaspaucimobilisDN-5. In this study, both bacterial strains were used to check plant growth-promoting activities like the production of IAA, ammonia, hydrogen cyanide, siderophore, phosphate solubilization, etc. which is important for the plant growth promotion, soil fertility, and development of eco-friendly sustainable agriculture. Later, both bacterial strains were also used for the biodegradation of the pesticides contaminated soil, and degradation was analyzed by HPLC. Based on plant growth-promoting activities S. paucimobilis DN-5 was selected for the growth experiment on Vigna radiata.