The emergence of multi-antibiotic resistant microorganisms is a major threat to public health. The search for alternative molecules to the use of classical antibiotics has become a necessity. The present work explores the possibility of using bioactive substances produced by lactic acid bacteria isolated from the gastrointestinal tract of quail, giving them the ability to combat resistant pathogens. To this end, microbiological analysis of quail caeca
on MRS agar for lactic acid bacteria isolates was carried out. Subsequently, direct and indirect confrontation tests carried out on solid and liquid MRS media (Difco, USA) against pathogenic strains of Escherichia coli
, Staphylococcus aureus
and Salmonella enterica
serogroup O:8, by means of lactic acid bacteria isolates, revealed their potential antibacterial activity. Of the 29 bacterial isolates obtained after microbiological analysis, ten (10) lactic acid bacterial isolates showed excellent antibacterial potential against the target pathogens, with zones of inhibition ranging from 3.5±0 to 25.5 ±0.5 mm. The lactic acid bacterial isolates C6S3, C4S2, C6S4 and C13S4 proved to be holders of substances with antagonistic activity against the growth of the 3 target pathogens, with diameters of the inhibition zones varying from 5.5 ± 1 to 13 ± 0.5 mm for the Escherichia coli strain, from 4 ± 0 to 13 ± mm for the Salmonella
serogroup O: 8 and from 3.5 ± 0.5 to 7 ± 0 mm for the Staphyloccocus aureus
strain. Therefore, the suspicion of a plausible presence of bioactive substances with antibacterial characteristics opens up avenues of exploration of the extracellular content of these isolates, with the simple aim of listing potential constituents that could be excellent alternatives in the fight against multidrug resistance to antibiotics.