Phomopsis viticola causes leaf spot disease to the Grapes plant is a potential pathogen of Grapes plant (Vitis Vinifera), was isolated from diseased Vitis Vinifera leaves from Nashik district and used for the present study. Pathogen was grown on the Czapek-Dox liquid medium substituting or adding different carbon, nitrogen to study cellulolytic and pectolytic enzyme production and total phenol production. The activity of enzyme was observed on the 8th day of incubation period. A grate extent of growth variation was observed on different carbon, nitrogen. Among the carbon source, maximum growth on Glucose followed by Control, lactose, fructose and dextrose while in absence of carbon source there was no growth. From nitrogen source maximum growth on nickel nitrate. While barium nitrate, cobalt nitrate and control had more or less similar effect on the growth. The least growth was observed in potassium nitrate. In absence of nitrogen source in the medium resulted in lowest growth as compared to other compound. Nitrogen sources shows much variation in cellulolytic enzyme activity. The cellulose activity was maximum in nickel nitrate and minimum in barium nitrate and potassium nitrate as compare to cobalt nitrate and control.