The use of silver nanoparticles (Ag-NPs) is rapidly increasing, but there are limited data on their effects on the aquatic environment. The present study aimed to determine the acute toxicity and evaluate the effect of subacute concentrations of Ag-NPs on hematological and plasma biochemical indices of, Roho, Labeo rohita
. The 24-, 48-, 72- and 96-h median lethal concentration values of silver Nanoparticle for Labeo rohita
were estimated at 5mg/l, 10 mg/l and 20 mg/L. Red blood cell (RBC) count, hemoglobin (Hb) count and hematocrit (Hct) level were significantly reduced at both concentrations tested (p < 0.05). In conclusion, Ag-NPs intoxication resulted in erythrocyte reduction, hematological disturbances, leucocytosis and stress response in Labeo rohita
and offered a simple tool to evaluate toxicity-derived alterations.