Phylogenetic analysis of cotton leaf curl CLCuD virus from old world to new world
Aqsa Tahir, Muhammad Mubin, M Shah Nwaz ul Rehman, Tahira Kabeer, Ammara Sana, Raheela Qayyum, Javaria Tabusam
In Pakistan Cotton is one of the mainly significant fiber and cash crop. CLCuD affect the cotton production mainly in Pakistan. The disease of cotton leaf curl is due to multiple viruses from genus begomovirus and geminiviradae family. Viruses transmitted by whitefly and have single stranded DNA genome. CLCD is a synergistic interaction between virus and linked satellite molecules include betasatellites and alphasatellites. Viruses of different plant develop by mutation and recombination. The particular satellite molecule CLCuMuB is pathogenicity determinant to create disease in cotton crop. In this analysis detailed analysis of phylogenetic of cotton leaf curl betasatellite infect cotton was performed. Sequences of Cotton leaf curl betasatellites retrieved from NCBI database then align with the use of software ClustalW and construct phylogenetic tree through neighbor joining method by using MEGA 7 to know evolutionary relations between many strains of cotton leaf curl betasatellite. Percentage identity in column was achieved by run sequences in SDT. At the last part nucleotide BLAST tool used for sequences have similarity lower than 90 was performed to observe similarity and difference among sequences of cotton leaf curl betasatellite.